“Chromosome Spots and Microtubule Dynamics”

  Budding yeast chromosome structure and dynamics during mitosis

Centromere Proximal spots exhibited dynamic separation and oscillations along the preanaphase spindle (Figure 2 Pearson et al., 2001)

Centromere dynamics of all chromosomes using the pan-specific centromere marker Cse4p fused to GFP.

Anaphase A centromere movements to the spindle pole using the 1.1kb CEN11 marker and Nuf2-GFP to label the spindle pole bodies.

Centromere movement to the pole using Cse4-GFP to label all the centromeres.

Fluorescent tufts of kinetochore microtubules shortening to the pole.

Visualization of astral microtubules using dynein-GFP.