ASH1 mRNA Localization in 3 Acts
Dale L. Beach and Kerry Bloom
Figure 7: GFP-Yta6 localizes to the maternal cortexGFP-Yta6 in Wildtype (YEF473a) cells. Video Sequence 9.
Spots of GFP-Yta6 remain on the maternal cortex until late in the cell cycle. Anaphase onset can be observed via HistoneH4-CFP staining of the chromatin, and compared to the accumulation of GFP-Yta6 in the bud.
- Top/Left: brightfield
- Top/Right: GFP-Yta6
- Bottom/Left: Chromatin (HistoneH4-CFP)
- Bottom/Right: Composite of GFP + CFP
The localization of the GFP-YTA6-ASH1 mRNA and protein can be observd during the accumulation of Yta6-GFP-ASH1 fusion protein into the bud.