Publications
Journal Articles:
Mishra PK, Au WC, Castineira PG, Ali N, Stanton J, Boeckmann L, Takahashi Y, Costanzo M, Boone C, Bloom KS, Thorpe PH, Basrai MA. Misregulation of cell cycle dependent methylation of budding yeast CENP-A contributes to chromosomal instability. Mol Biol Cell. 2023 Jul 12:mbcE23030108. doi: 10.1091/mbc.E23-03-0108. Online ahead of print.
González L, Kolbin D, Trahan C, Jeronimo C, Robert F, Oeffinger M, Bloom K, Michnick SW. Adaptive partitioning of a gene locus to the nuclear envelope in Saccharomyces cerevisiae is driven by polymer-polymer phase separation. Nat Commun. 2023 Feb 28;14(1):1135. doi: 10.1038/s41467-023-36391-6.
Lawrimore J, de Larminat SC, Cook D, Friedman B, Doshi A, Yeh E, Bloom K. Polymer models reveal how chromatin modification can modulate force at the kinetochore. Mol Biol Cell. 2022 Sep 15;33(11):ar97. doi: 10.1091/mbc.E22-02-0041. Epub 2022 Jun 15.
Locatelli M, Lawrimore J, Lin H, Sanaullah S, Seitz C, Segall D, Kefer P, Salvador Moreno N, Lietz B, Anderson R, Holmes J, Yuan C, Holzwarth G, Bloom KS, Liu J, Bonin K, Vidi PA. DNA damage reduces heterogeneity and coherence of chromatin motions. Proc Natl Acad Sci U S A. 2022 Jul 19;119(29):e2205166119. doi: 10.1073/pnas.2205166119. Epub 2022 Jul 12.
Bloom K, Kolbin D. Mechanisms of DNA Mobilization and Sequestration. Genes (Basel). 2022 Feb 16;13(2):352. doi: 10.3390/genes13020352.
Lawrimore J, Bloom K. Shaping centromeres to resist mitotic spindle forces. J Cell Sci. 2022 Feb 15;135(4):jcs259532. doi: 10.1242/jcs.259532. Epub 2022 Feb 18.
He Y, Adalsteinsson D, Walker B, Lawrimore J, Forest MG, Bloom K. Simulating Dynamic Chromosome Compaction: Methods for Bridging In Silico to In Vivo. Methods Mol Biol. 2022;2415:211-220. doi: 10.1007/978-1-0716-1904-9_16.
Mishra PK, Wood H, Stanton J, Au WC, Eisenstatt JR, Boeckmann L, Sclafani RA, Weinreich M, Bloom KS, Thorpe PH, Basrai MA. Cdc7-mediated phosphorylation of Cse4 regulates high-fidelity chromosome segregation in budding yeast. Mol Biol Cell. 2021 Nov 1;32(21):ar15. doi: 10.1091/mbc.E21-06-0323. Epub 2021 Aug 25.
Lawrimore J, Kolbin D, Stanton J, Khan M, de Larminat SC, Lawrimore C, Yeh E, Bloom K. The rDNA is biomolecular condensate formed by polymer-polymer phase separation and is sequestered in the nucleolus by transcription and R-loops. Nucleic Acids Res. 2021 May 7;49(8):4586-4598. doi: 10.1093/nar/gkab229.
Kefer P, Iqbal F, Locatelli M, Lawrimore J, Zhang M, Bloom K, Bonin K, Vidi PA, Liu J. Performance of deep learning restoration methods for the extraction of particle dynamics in noisy microscopy image sequences. Mol Biol Cell. 2021 Apr 19;32(9):903-914. doi: 10.1091/mbc.E20-11-0689. Epub 2021 Jan 27.
Cook D, Long S, Stanton J, Cusick P, Lawrimore C, Yeh E, Grant S, Bloom K. Behavior of dicentric chromosomes in budding yeast. PLoS Genet. 2021 Mar 18;17(3):e1009442. doi: 10.1371/journal.pgen.1009442. eCollection 2021 Mar.
Mishra PK, Chakraborty A, Yeh E, Feng W, Bloom KS, Basrai MA. R-loops at centromeric chromatin contribute to defects in kinetochore integrity and chromosomal instability in budding yeast. Mol Biol Cell. 2021 Jan 1;32(1):74-89. doi: 10.1091/mbc.E20-06-0379. Epub 2020 Nov 4.
He Y, Lawrimore J, Cook D, Van Gorder EE, De Larimat SC, Adalsteinsson D, Forest MG, Bloom K. Statistical mechanics of chromosomes: in vivo and in silico approaches reveal high-level organization and structure arise exclusively through mechanical feedback between loop extruders and chromatin substrate properties. Nucleic Acids Res. 2020 Nov 18;48(20):11284-11303. doi: 10.1093/nar/gkaa871.
Lawrimore CJ, Lawrimore J, He Y, Chavez S, Bloom K. Polymer perspective of genome mobilization. Mutat Res. 2020 May-Dec;821:111706. doi: 10.1016/j.mrfmmm.2020.111706. Epub 2020 May 26.
Lawrimore CJ, Bloom K. Common Features of the Pericentromere and Nucleolus. Genes (Basel). 2019 Dec 10;10(12):1029. doi: 10.3390/genes10121029.
Lawrimore J, Doshi A, Walker B, Bloom K. AI-Assisted Forward Modeling of Biological Structures. Front Cell Dev Biol. 2019 Nov 14;7:279. doi: 10.3389/fcell.2019.00279. eCollection 2019.
Walker B, Taylor D, Lawrimore J, Hult C, Adalsteinsson D, Bloom K, Forest MG. Transient crosslinking kinetics optimize gene cluster interactions. PLoS Comput Biol. 2019 Aug 21;15(8):e1007124. doi: 10.1371/journal.pcbi.1007124. eCollection 2019 Aug.
Lawrimore J, Bloom K. The regulation of chromosome segregation via centromere loops. Crit Rev Biochem Mol Biol. 2019 Aug;54(4):352-370. doi: 10.1080/10409238.2019.1670130. Epub 2019 Oct 1.
Hamdani O, Dhillon N, Hsieh TS, Fujita T, Ocampo J, Kirkland JG, Lawrimore J, Kobayashi TJ, Friedman B, Fulton D, Wu KY, Chereji RV, Oki M, Bloom K, Clark DJ, Rando OJ, Kamakaka RT. tRNA Genes Affect Chromosome Structure and Function via Local Effects. Mol Cell Biol. 2019 Apr 2;39(8):e00432-18. doi: 10.1128/MCB.00432-18. Print 2019 Apr 15.
Lawrimore J, He Y, Forest GM, Bloom K. Three-Dimensional Thermodynamic Simulation of Condensin as a DNA-Based Translocase. Methods Mol Biol. 2019;2004:291-318. doi: 10.1007/978-1-4939-9520-2_21.
Cook DM, Bennett M, Friedman B, Lawrimore J, Yeh E, Bloom K. Fork pausing allows centromere DNA loop formation and kinetochore assembly. Proc Natl Acad Sci U S A. 2018 Nov 13;115(46):11784-11789. doi: 10.1073/pnas.1806791115. Epub 2018 Oct 29.
Lawrimore J, Doshi A, Friedman B, Yeh E, Bloom K. Geometric partitioning of cohesin and condensin is a consequence of chromatin loops. Mol Biol Cell. 2018 Nov 1;29(22):2737-2750. doi: 10.1091/mbc.E18-02-0131. Epub 2018 Sep 12.
Lianga N, Doré C, Kennedy EK, Yeh E, Williams EC, Fortinez CM, Wang A, Bloom KS, Rudner AD. Cdk1 phosphorylation of Esp1/Separase functions with PP2A and Slk19 to regulate pericentric Cohesin and anaphase onset. PLoS Genet. 2018 Mar 21;14(3):e1007029. doi: 10.1371/journal.pgen.1007029. eCollection 2018 Mar.
Bloom K. Cell Division: Single-Cell Physiology Reveals Secrets of Chromosome Condensation. Curr Biol. 2018 Feb 5;28(3):R117-R119. doi: 10.1016/j.cub.2017.12.032.
Bloom K. Liberating cohesin from cohesion. Genes Dev. 2017 Nov 1;31(21):2113-2114. doi: 10.1101/gad.309732.117. Review.
Haase KP, Fox JC, Byrnes AE, Adikes RC, Speed SK, Haase J, Friedman B, Cook DM, Bloom K, Rusan NM, Slep KC. Stu2 uses a 15-nm parallel coiled coil for kinetochore localization and concomitant regulation of the mitotic spindle. Mol Biol Cell. 2018 Feb 1;29(3):285-294. doi: 10.1091/mbc.E17-01-0057. Epub 2017 Nov 29.
Lawrimore J, Friedman B, Doshi A, Bloom K. RotoStep: A Chromosome Dynamics Simulator Reveals Mechanisms of Loop Extrusion. Cold Spring Harb Symp Quant Biol. 2017 Nov 22. pii: 033696. doi: 10.1101/sqb.2017.82.033696.
Hult C, Adalsteinsson D, Vasquez PA, Lawrimore J, Bennett M, York A, Cook D, Yeh E, Forest MG, Bloom K. Enrichment of dynamic chromosomal crosslinks drive phase separation of the nucleolus. Nucleic Acids Res. 2017 Nov2;45(19):11159-11173. doi: 10.1093/nar/gkx741.
Bloom K, Costanzo V. Centromere Structure and Function. Prog Mol Subcell Biol. 2017;56:515-539. doi: 10.1007/978-3-319-58592-5_21.
Takada M, Zhang W, Suzuki A, Kuroda TS, Yu Z, Inuzuka H, Gao D, Wan L, Zhuang M, Hu L, Zhai B, Fry CJ, Bloom K, Li G, Karpen GH, Wei W, Zhang Q. FBW7 Loss Promotes Chromosomal Instability and Tumorigenesis via Cyclin E1/CDK2-Mediated Phosphorylation of CENP-A. Cancer Res. 2017 Sep 15;77(18):4881-4893. doi: 10.1158/0008-5472.CAN-17-1240. Epub 2017 Jul 31.
Salmon ED, Bloom K. Tension sensors reveal how the kinetochore shares its load. Bioessays. 2017 Jul;39(7). doi: 10.1002/bies.201600216. Epub 2017 Jun 5. Review.
Lawrimore J, Barry TM, Barry RM, York AC, Friedman B, Cook DM, Akialis K, Tyler J, Vasquez P, Yeh E, Bloom K. Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage. Mol Biol Cell. 2017 Jun 15;28(12):1701-1711. doi: 10.1091/mbc.E16-12-0846. Epub 2017 Apr 27.
Vasquez, P.A., Caitlin Hult, David Adalsteinsson, Josh Lawrimore, M Gregory Forest, and Kerry Bloom (2016) Entropy gives rise to topological associated domains Nucleic Acids Research, (in press)
Mishra PK, Ciftci-Yilmaz S, Reynolds D, Au WC, Boeckmann L, Dittman LE, Jowhar ZJ, Pachpor T, Yeh E, Baker RE, Hoyt MA, D’Amours D, Bloom K, Basrai MA (2016) Polo kinase Cdc5 associates with centromeres to facilitate the removal of centromeric cohesin during mitosis Mol Biol Cell. May 25. pii: mbc.E16-01-0004. [Epub ahead of print].
Tsabar, M., Haase, J., Harrison, B., Snider, C.E., Eldridge, B., Kaminsky, L., Hine R.M., Haber, J.E., and Bloom, K. (2016) A cohesin-based partitioning mechanism revealed upon transcriptional inactivation of centromere PLOS Genetics Apr 29;12(4):e1006021.
Falk, J.E., Tsuchiya, D., Verdaasdonk, J., Lacefield, S., Bloom, K. and Amon, A. (2016) Spatial signals link exit from mitosis to spindle position eLIFE. May 11;5. pii: e14036 doi: 10.7554/eLife.14036
Suzuki, A., Badger, B.L., Haase, J., Ohashi, T., Erickson, H.P., Salmon, E.D., Bloom , K. (2016) How the kinetochore couples microtubule force and centromere stretch to move chromosomes Nature Cell Biology 18:382-392. PMID: 26974660
Ohkuni K, Takahashi Y, Fulp A, Lawrimore J, Au WC, Pasupala N, Levy-Myers R, Warren J, Strunnikov A, Baker RE, Kerscher O, Bloom K, Basrai MA (2016) SUMO-Targeted Ubiquitin Ligase (STUbL) Slx5 regulates proteolysis of centromeric histone H3 variant Cse4 and prevents its mislocalization to euchromatin. Mol Biol Cell. Mar 9. pii: mbc.E15-12-0827. [Epub ahead of print] PMID: 26960795
Stephens AD, Snider CE, Bloom K. (2015) The SUMO deconjugating peptidase Smt4 contributes to the mechanism required for transition from sister chromatid arm cohesion to sister chromatid pericentromere separation. Cell Cycle. May 6:0
Calderon CP, Bloom K. (2015) Inferring Latent States and Refining Force Estimates via Hierarchical Dirichlet Process Modeling in Single Particle Tracking Experiments PLoS One. 2015 Sep 18;10(9):e0137633. doi: 10.1371/journal.pone.0137633. eCollection 2015
Lawrimore, J., Aicher, J., Hahn, P., Fulp, A., Kompa, B., Vicci, L., Falvo, M., Taylor, R.M., Bloom, K. (2015). ChromoShake: A chromosome dynamics simulator reveals that chromatin loops stiffen centromeric chromatin. Molecular Biology of the Cell, 153-166. Program Source Code
Lawrimore J, Vasquez PA, Falvo MR, Taylor RM 2nd, Vicci L, Yeh E, Forest MG, Bloom K. (2015) DNA loops generate intracentromere tension in mitosis. Journal of Cell Biology 210:553-564
Snider, C.E., Stephens, A.D., Kirkland, J.G., Hamdani, O., Kamakaka, R.T., and Bloom, K. (2014) Dyskerin, tRNA genes, and condensin tether pericentric chromatin to the spindle axis in mitosis.Journal of Cell Biology 207: 189-199
Verdaasdonk, J.S., Vasquez, P.A., Barry, R.M., Barry, T., Goodwin, S., Forest, M.G., and Bloom, K. (2013) Centromere Tethering Confines Chromosome Domains Molecular Cell 52: 819-831
Ren, J., Lin, C., Pathak, M., Temple, B., Nile, A., Mousley, C., Duncan, M., Eckert, D., Leiker, T.,Ivanova, P., Myers, D., Murphy, R., Brown, H., Verdaasdonk, J., Bloom, K.S., Ortlund, E., Neiman, A., and Bankaitis, V. (2013) A phosphatidylinositol transfer protein integrates phosphoinositide signaling with lipid droplet metabolism to regulate a developmental program of nutrient stress-induced membrane biogenesis Molecular Biology of the Cell 25: 712-727
Stephens, A.D., Snider, C.E., Haase, J., Haggerty, R.A., Vasquez, P.A., Forest, M.G., and Bloom, K. (2013) Individual pericentromeres display coordinated motion and stretching in the yeast spindle Journal of Cell Biology 203:407-416
Song, W., Gawel, M., Dominska, M., Greenwell, P.W., Hazkani-Covo, E., Bloom, K., and Petes, T.D. (2013) Nonrandom Distribution of Interhomolog Recombination Events Induced by Breakage of aDicentric Chromosome in Saccharomyces cerevisiae Genetics 194: 69-80
Stephens, A.D., Quammen, C., Chang, B., Haase, J., Taylor II, R.M., and Bloom, K. (2013) The spatial segregation of pericentric cohesin and condensin in the mitotic spindle Molecular Biology of the Cell 24: 3909-3919
Haase, J., Mishra, P., Stephens, A.D., Haggerty, R., Quammen, C., Taylor II, R.M., Yeh, E., Basrai, M., and Bloom, K. (2013) A 3D Map of the Yeast Kinetochore Reveals the Presence of Core and Accessory Centromere-Specifice Histone Current Biology 23: 1939-1944
Haber, J., Braberg, H., Wu, Q., Alexander, R., Haase, J., Ryan, C., Lipkin-Moore, Z., Franks-Skiba, K., Johnson, T., Shales, M., Lenstra, T., Holstege, F., Johnson, J., Bloom, K., and Krogan, N. (2013)Systematic Triple-Mutant Analysis Uncovers Functional Connectivity between Pathways Involved in Chromosome Regulation Cell Reports 3:1-11
Stephens, A.D., Haggerty, R.A., Vasquez, P.A., Vicci, L., Snider, C.E., Shi, F., Quammen, C., Mullins, C., Haase, J., Taylor, R.M., Verdaasdonk, J.S., Falvo, M.R., Jin, Y., Forest, M.G., and Bloom, K. (2013)Pericentric chromatin loops function as a nonlinear spring in mitotic force balance Journal of Cell Biology 200:757-772
Verdaasdonk, J.S., Gardner, R., Stephens, A.S., Yeh, E., and Bloom, K. (2012) Tension-dependent nucleosome remodeling at the pericentromere in yeast Molecular Biology of the Cell 23: 2560-2570
Haase, J., Stephens, A.D., Verdaasdonk, J.S., Yeh, E., and Bloom, K. (2012) Bub1 Kinase and Sgo1 Modulate Pericentric Chromatin in Response to Altered Microtubule Dynamics Current Biology 22: 471-481
Stephens, A.D., Haase, J., Vicci, L., Taylor II, R.M., and Bloom, K. (2011) Cohesin, condensin, and the intramolecular centromere loop together generate the mitotic chromatin spring Journal of Cell Biology 193:1167-1180
Lawrimore, J., Bloom, K.S., and Salmon, E.D. (2011) Point centromeres contain more than a single centromere-specific Cse4 (CENP-A) nucleosome Journal of Cell Biology 195:573-582
Li, Z., Vizeacoumar, F.J., Bahr, S., Li, J., Warringer, J., Vizeacoumar, F.S., Min, R., VanderSluis, B., Bellay, J., DeVit, M., Fleming, J.A., Stephens, A., Haase, J., Lin, Z.Y., Baryshnikova, A., Lu, H., Yan, Z., Jin, K., Barker, S., Datti, A., Giaever, G., Nislow, C., Bulawa, C., Myers, C., Costanza, M., Gingras, A.C., Zhang, Z., Blomberg, A., Bloom, K., Andrews, B., and Boone, C. (2011) Systematic exploration of essential yeast gene function with temperature-sensitive mutants Nature Biotechnology 29:361-369
Larson, M., Harrison, B., and Bloom, K. (2010) Uncovering chromatin’s contribution to the mitotic spindle: Applications of computational and polymer models Biochimie 92:1741-1748
Nakai, W., Westmoreland, J., Yeh, E., Bloom, K., and Resnick, M.A. (2010) Chromosome integrity at a double-strand break requires exonuclease 1 and MRX DNA Repair 10:102-110
Vizeacoumar, F.J., van Dyk, N., Vizeacoumar, F.S., Cheung, V., Li, J., Sydorskyy, Y., Case, N., Li, Z.,Datti, A., Nislow, C., Raught, B., Zhang, Z., Frey, B., Bloom, K., Boone, C., and Andrews, B. (2010)Integrating high-throughput genetic interaction mapping and high-content screening to explore yeast spindle morphogenesis Journal of Cell Biology 188:69-81.
Anderson, M., Haase, J., Yeh, E., and Bloom, K. (2009) Function and Assembly of DNA looping, Clustering, and Microtubule Attachment Complexes within a Eukaryotic Kinetochore Molecular Biology of the Cell 20:4131-4139.
Harrison, B., Hoang, M., and Bloom, K. (2009) Persistent mechanical linkage between sister chromatids throughout anaphase Chromosoma 118:633–645
Joglekar, A., Bloom, K., and Salmon, E.D. (2009) In Vivo Protein Architecture of the Eukaryotic Kinetochore with Nanometer Scale Accuracy Current Biology 19:694-699.
Fisher, J.K., Ballenger, M., O’Brien, E.T., Haase, J., Superfine, R., and Bloom, K. (2009) DNA relaxation as a probe for the intracellular environment PNAS 106:9250-9255.
Gardner, M., Bouck, D., Paliulis, L., Meehl, J., O’Toole, E., Haase, J., Soubry, A., Joglekar, A., Winey, M., Salmon, E.D., Bloom, K., and Odde, D.(2008) Chromosome congression by Kinesin-5 motor-mediated disassembly of longer kinetochore microtubules Cell 135:894-906.
Quammen, C.W., Richardson, A.C., Haase, J., Harrison, B.D., Taylor II, R.M., and Bloom, K. (2008)Fluorosim: A Visual Problem-Solving Environment for Fluorescence Microscopy EurographicsWorkshop on Visual Computing for Biomedicine
Gardner, M., Odde, D., and Bloom, K. (2008) Kinesin-8 molecular motors: putting the brakes on chromosome oscillations Trends in Cell Biology 18:307-310.
Bouck, D., Joglekar, A., and Bloom, K. (2008) Design Features of a Mitotic Spindle: Balancing Tension and Compression at a Single Microtubule Kinetochore Interface in Budding Yeast Annual Review of Genetics 361:13.1-13.25.
Joglekar, A., Bouck, D., Finley, K., Liu, X., Wan, Y., Berman, J., He, X., Salmon, E.D., and Bloom, K. (2008) Molecular architecture of the kinetochore-microtubule attachment site is conserved between point and regional centromeres Journal of Cell Biology 181:587-594.
Yeh, E., Haase, J., Paliulis, L., Joglekar, A., Bond, L., Bouck, D., Salmon, E.D., and Bloom, K.(2008) Pericentric Chromatin Is Organized into an Intramolecular Loop in Mitosis. Current Biology 18:81-90.
Gardner, M., Haase, J., Karthikeyan, M., Molk, J., Anderson, M., Joglekar, A., O’Toole, E., Winey, M., Salmon, E.D., Odde, D., and Bloom, K. (2008) The microtubule-based motor Kar3 and plus end–binding protein Bim1 provide structural support for the anaphase spindle Journal of Cell Biology 180:91-100.
Gardner, M., Odde, D., and Bloom, K. (2007) Hypothesis testing via integrated computer modeling and digital fluorescence microscopy Methods 41:232-237
Bouck, D.C. and Bloom K. (2007) Pericentric chromatin is an elastic component of the mitotic spindle. Curr. Biology 17:1-8
Dotiwala, F., Haase, J., Arbel-Eden, A., Bloom, K., and Haber, J. (2007) The yeast DNA damage checkpoint proteins control a cytoplasmic response to DNA damage. PNAS 104: 11358-11363
Shimogawa M.M., Graczyk, B., Gardner, M.K., Francis, S.E., Ess, M., Ruse, C., Niessen, S., Muller, E., Yoder, T.J., Molk, J.N, Bloom, K., Yates, J.R., III, Odde, D.J., and Davis, T.N. (2006) Mps1 Phosphorylation of the Kinetochore Component Dam1 Couples Kinetochores to Microtubule Plus Ends at Metaphase Current Biology 16:1489–1501.
Pearson C.G., Gardner M.K., Paliulis L.V., Salmon E.D., Odde D.J., and Bloom K. (2006)
Measuring Nanometer Scale Gradients in Spindle Microtubule Dynamics Using Model Convolution Microscopy Mol. Biol. Cell 17:4069-4709.
Joglekar, A.P., Bouck, D.C., Molk, J.N, Bloom, K.S., and Salmon, E.D. (2006) Molecular Architecture of a Kinetochore-microtubule attachment site Nature Cell Biology 8:581-585.
Fisher, J.K., Cribb, J., Desai, K.V., Vicci, L., Wilde, B., Keller, K., Taylor II, R.M., Haase, J., Bloom, K., O’Brien, E.T., and Superfine, R. (2006) Thin-foil magnetic force system for high-numerical-aperture microscopy Review of Scientific Instruments 77:023702.1-023702.9.
Bloom, K., Sharma, Shantanu, and Dokholyan, Nikolay V. (2006) The path of DNA in the kinetochore Current Biology 16:R276-R278.
Molk, J.N., Salmon, E.D., and Bloom, K. (2006) Nuclear congression is driven by cytoplasmic microtubule plus end interactions in S. cerevisiae Journal of Cell Biology 172:27-39.
Gardner, M.K., Pearson, C.G., Sprague, B.L., Zarzar, T.R., Bloom, K., Salmon, E.D., and Odde, D.J. (2005) Tension-Dependent Regulation of Microtubule Dynamics at Kinetochores Can Explain Metaphase Congression in Yeast Molecular Biology of the Cell 16:3764-3775.
Jones, M.H., Huneycutt, B.J., Pearson, C.G., Zhang, C., Morgan, G., Shokat, K., Bloom, K., and Winey, M. (2005) Chemical Genetics Reveals a Role for Mps1 Kinase in Kinetochore Attachment during Mitosis Current Biology 15:160-165.
Bouck, David C. and Bloom, Kerry S. (2005) The kinetochore protein Ndc10p is required for spindle stability and cytokinesis in yeast PNAS 102:5408–5413.
Lobachev, K., Vitriol, E., Stemple, J., Resnick, M.A., Bloom, K. (2004) Chromosome Fragmentation after Induction of a Double-Strand Break Is an Active Process Prevented by the RMX Repair Complex Current Biology 14:2107-2112.
Pearson CG, Yeh E, Gardner M, Odde D, Salmon ED, Bloom K (2004) Stable kinetochore-microtubule attachment constrains centromere positioning in metaphase Current Biology 14:1962-1967.
Molk, J., Schuyler, S., Liu, J., Evans, J., Salmon, E.D., Pellman, D., and Bloom, K. (2004) The Differential Roles of Budding Yeast Tem1p, Cdc15p, and Bub2p Protein Dynamics in Mitotic Exit Mol. Biol. Cell 15:1519-1532.
Maddox, P.S., Stemple, J., Satterwhite, L., Salmon, E.D., and Bloom, K. (2003) The minus-end directed motor, Kar3, is required for coupling dynamic microtubule plus ends to the cortical shmoo tip in budding yeast. Current Biology 19:1423-1428.
Pearson, C.G., Maddox, P.S., Zarzar, T., Salmon, E.D., and Bloom, K. (2003) Yeast kinetochores do not stabilize Stu2 dependent spindle microtubule dynamics. Mol. Biol. Cell 14:4181-4195
Tennessee J. Yoder, Chad G. Pearson, Kerry Bloom, and Trisha N. Davis (2003) The Saccharomyces cerevisiae spindle pole body is a dynamic structure. Mol. Biol. Cell 14: 3494-3505
Brian L.Sprague, Chad G.Pearson, Paul S.Maddox, Kerry S.Bloom, E.D.Salmon, and David.J.Odde (2003) Mechanisms of Microtubule-Based Kinetochore Positioning in the Yeast Metaphase Spindle Biophysical Journal 84: 3529 –3546
Mythreye, K., and Bloom, K.S. (2003) Differential kinetochore protein requirements for establishment versus propagation of centromere activity in Saccharomyces cerevisiae. J. Cell Biol 160: 833-843
Thrower, Douglas, A., Stemple, J., Yeh, E. and Bloom, K. (2003) Nuclear oscillations and nuclear filament formation accompany single-strand annealing repair of a dicentric chromosome in Saccharomyces cerevisiae. Journal of Cell Science 116: 561-569
Segal, M., Bloom, K., and Reed S.I. (2002) Kar9p-independent microtubule capture at Bud6p cortical sites primes spindle polarity prior to bud emergence in S. cerevisiae. Mol. Biol. Cell. 13: 4141-4155
Gupta, M.L. Jr., Bode, C.J., Pearson, C.G., Thrower, D.A., Suprenant, K.A., Bloom, K.S., and Himes R.H. (2002) Tubulin C354 mutations that decrease microtubule dynamics do not prevent nuclear migration. Mol. Biol. Cell. 13:2919-32
Jacalyn Vogel, Ben Drapkin, Jamina Oomen, Dale Beach, Kerry Bloom, and Michael Snyder (2001) Phosphorylation of [gamma]-Tubulin Regulates Microtubule Organization in Budding Yeast. Dev. Cell 1(5):621-631.
Karena A. Kosco, Chad G. Pearson, Paul S. Maddox, Peijing Jeremy Wang, Ian R. Adams, E. D. Salmon, Kerry Bloom, and Tim C. Huffaker (2001) Control of Microtubule Dynamics by Stu2p Is Essential for Spindle Orientation and Metaphase Chromosome Alignment in Yeast Mol. Biol. Cell.12: 2870-2880.
Dale L. Beach and Kerry Bloom. (2001) ASH1 mRNA Localization in Three Acts Mol. Biol. Cell. 12: 2567-2577. Related Movies
Pearson, C., Maddox, P., Salmon, E.D. and Bloom. K. (2001) Budding yeast chromosome structure and dynamics during mitosis. J. Cell Biology 152: 1255-1266.
Movies: Figure 2, Figure 3, Figure 4a, Figure 4c, Figure 4e
Douglas A. Thrower and Kerry Bloom (2001) Dicentric Chromosome Stretching during Anaphase Reveals Roles of Sir2/Ku in Chromatin Compaction in Budding Yeast. Mol. Biol. Cell. 12: 2800-2812.Related Movies
Maddox, P.S., Bloom, K.S. and E.D. Salmon (2000) The polarity and dynamics of microtubule assembly in the budding yeast Saccharomyces cerevisiae. Nature Cell Biology 2:36-41.
Segal, M., Maddox, P.S., Salmon, E.D., Bloom, K.S. and Reed, S.I. (2000) Establishment of spindle polarity requires Clb5-dependent kinase in budding yeast. J. Cell Biol. 148:441-451.
Elaine Yeh, Charlie Yang, Elaine Chin, Paul Maddox, E. D. Salmon, Daniel J. Lew, and Kerry Bloom. (2000) Dynamic Positioning of Mitotic Spindles in Yeast:. Role of Microtubule Motors and Cortical Determinants Mol. Biol. Cell 11: 3949-3961. Figure 5, Figure 7
Marisa Segal, Kerry Bloom, Steven Reed. (2000) Bud6 Directs Sequential Microtubule Interactions with the Bud Tip and Bud Neck during Spindle Morphogenesis in Saccharomyces cerevisiae. Mol. Biol. Cell 11: 3689-3702.
Beach, D.L., Thibodeaux, J., Maddox, P., Yeh, E., Bloom. K. (2000) The role of the proteins Kar9 and Myo2 in orienting the mitotic spindle of budding yeast. Current Biology 10(23):1497-1506.
Maddox, P., Chin, E., Mallavarapu, A., Yeh, E., Salmon, E.D., Bloom, K. (1999) Microtubule dynamics from mating through the first zygotic division in the budding yeast Saccharomyces cerevisiae. J. Cell Biol. 144:977-987.
Beach, D.L., Salmon, E.D., and Bloom, K. (1999) Localization and anchoring of mRNA in budding yeast Current Biology 9:569-578.
Maddox, P. (1999) Researchers Find that mRNA is Actually Green.-not affiliated with THE ONION.
Theesfeld, C., Irazoqui, J.E., Bloom, K., and Lew, D.J. (1999). The role of actin in spindle orientation changes during the S. cerevisiae cell cycle. J. Cell Biol. 146:1019-1032.
Shaw, S.L., Maddox, P., Skibbens, R.V., Yeh, E., Salmon, E.D. and Bloom, K. (1998) Nuclear and spindle dynamics in budding yeast. Mol. Biol. Cell 9:1627-1631.
Yang, S., Yeh, E., Salmon, E.D. and Bloom K.S. (1997) Identification of a Mid-Anaphase Checkpoint in Yeast. J. Cell Biol. 136:345-354.
Shaw, S.L., Yeh, E., Bloom, K., and Salmon, E.D. (1997). Imaging green fluorescent protein fusion proteins in Saccharomyces cerevisiae. Current Biology 7:701-704.
Shaw, S.L., Yeh, E., Maddox, P., Salmon, E.D. and Bloom, K. (1997) Astral microtubule dynamics in yeast: A microtubule-based searching mechanism for spindle orientation and nuclear migration in the bud. J. Cell Biol. 139:985-994.
Yeh, E., Skibbens, R., Cheng, J., Salmon, E.D., and Bloom, K. (1995) Spindle dynamics and cell cycle regulation of cytoplasmicdynein in the yeast, S. cerevisiae . J. Cell Biol. 130:687-700.
Brock J. and Bloom, K. (1994) A chromosome breakage assay to monitor mitotic forces in budding yeast. J. Cell Science 107: 891-902.
Kramer, K.M., Brock, J.A., Bloom, K.S., Moore, J.K., and Haber, J.E. (1994). Two different types of double strand breaks in S. cerevisiae are repaired by similar RAD52 non-homologous recombination events. Mol. Cell Biol. 14:1293-1301.
Schulman, I. and Bloom, K. S. (1993). Genetic Dissection of Centromere Assembly. Mol.Cell Biol.13: 3156-66.
Heus, J.J., Zonneveld, B.J.M., Bloom, K.S., Steensma, H.Y. and van den Berg, J.A. (1993).The nucleosome repeat length of K. lactis is 16 bp longer than that of S. cerevisiae. NAR 21:2247-2248.
Li, Y.-Y., Yeh, E., Hays, T., and Bloom, K. (1993). Disruption of Mitotic spindle orientation in a Yeast Dynein mutant. Proc. Natl. Acad. Sci. USA 90:10096-10100.
Resnick, M.A., Westmoreland, J., and Bloom, K. (1990). Heterogeneity and maintenance of centromere plasmid copy number in S. cerevisiae. Chromosoma 99:281-288.
Saunders, M.A., Grunstein, M. and Bloom, K.S. (1990). Nucleosome Depletion Alters the Chromatin Structure of a Yeast Centromere. Molec Cell. Biology 10: 5721-5727.
Yeh, E., Driscoll, R., Coltrera, M., Olins, A. and Bloom, K. (1990). A dynamin like protein encoded by the yeast sporulation gene SPO15. Nature 349: 713-715.
Hill, A., and Bloom, K.S. (1989) The Acquisition and Processing of Dicentric Chromosomes in Yeast.Molecular and Cellular Biology 9:1368- 1370.
Saunders, M.A., Fitzgerald-Hayes, M. and Bloom, K.S. (1988). Chromatin Structure of Altered Yeast Centromeres. Proc. Natl. Acad. Sci. USA 85:175-179.
Kenna, M., Amaya, E. and Bloom K.S. (1988). Selective Excision of the Centromere Chromatin Complex from Saccharomyces cerevisiae. J. Cell Biol. 107:9-15.
Hill, A., and Bloom, K. (1987). Genetic Manipulation of Centromere Function Molecular and Cellular Biology 7:2397-2405.
Yeh, E., Carbon, J. and Bloom, K. (1986). Tightly centromere-linked gene (SPO15) essential for meiosis in the yeast Saccharomyces cerevisiae. Molecular and Cell Biology. 6: 158-167.
Bloom, K.S., Amaya, E., Carbon, J., Clarke, L., Hill, A, and Yeh, E. (1984) Chromatin Conformation of Yeast Centromeres. J. Cell Biology. 99: 1559-1568.
Bloom, K.S. and Carbon, J. (1982) Yeast centromere DNA is in a unique and highly ordered structure in chromosomes and small circular mini-chromosomes. Cell 29: 305-317.
Book Chapters:
Verdaasdonk, J.S., Lawrimore, J., and Bloom, K. (2014) Determining absolute protein numbers by quantitative fluorescence microscopy Methods in Cell Biology 123: 347-365
Joglekar, A., Salmon, E.D., and Bloom, K.(2007) Counting Kinetochore Protein Numbers in Budding Yeast Using Genetically Encoded Fluorescent Proteins Methods in Cell Biology 85:127-151.
Salmon, E.D., Shaw, S.L., Waters, J., Waterman-Storer, C.M., Maddox, P.S., Yeh, E., and Bloom, K. (2003) A High Resolution Multimode Digital Microscope System. Methods in Cell Biology 56:185-215
Salmon, E.D., Shaw, S.L.,Waters, J., Waterman-Storer, C., Maddox, P.S., Yeh, E., and Bloom, K. (1998) A High-Resolution Multimode Digital Microscope System. Methods in Cell Biology 56:186-216.
Bloom, K., Beach, D.L, Maddox, P., Shaw, S.L., Yeh, E., and Salmon, E.D. (1998). Using GFP Fusion Proteins to Quantitate Microtubule and Spindle Dynamics in Budding Yeast. Methods in Cell Biology 61:369-383.
Monographs and Invited Reviews:
Vasquez, P., and Bloom, K. (2014) Polymer models of interphase chromosomes Nucleus 5:5: 376-390
Bloom, K. (2014) Centromeric Heterochromatin: The Primordial Segregation Machine Annual Review of Genetics 48: 457-484
Verdaasdonk, J.S., Stephens, A.D., Haase, J., and Bloom, K. (2014) Bending the Rules: WidefieldMicroscopy and the Abbe Limit of Resolution Journal of Cell Physiology 229(2): 132-138
Bloom, K. (2013) A Close Look at Wiggly Chromosomes Developmental Cell 25:330-332
Winey, M., and Bloom, K. (2012) Mitotic Spindle Form and Function Genetics 190:1197-1224
Verdaasdonk, J.S., and Bloom, K.(2011) Centromeres: unique chromatin structures that drive chromosome segregation Nature Reviews Cell and Molecular Biology 12: 320-332
Bloom K., and Yeh, E. (2010) Tension Management in the Kinetochore Current Biology 20: R1040-R1048
Joglekar, A., Bloom, K., and Salmon, E.D. (2010) Mechanisms of force generation by end-on kinetochore-microtubule attachments. Current Opinion in Cell Biology 22:57-67
Gatlin, J., and Bloom, K. (2010) Microtubule Motors in Eukaryotic Spindle Assembly and Maintenance Semin Cell Dev Biol doi:10.1016/j.semcdb.2010.01.015
Joglekar, A., and Bloom, K. (2010) Towards Building a Chromosome Segregation Machine Nature 463:446-456.
Bloom, K. (2008) Question and Answer Current Biology 18:R541-R543.
Bloom, K. (2007) Beyond the code: the mechanical properties of DNA as they relate to mitosis Chromosoma
Maher, B. (2007) Spring Theory Nature 448:984-986.
Bloom, K. (2006) NoCut: Cytokinesis in Check Cell 125:17-18.
Bloom, K. (2005) Chromosome Segregation: Seeing Is Believing Current Biology 15:R500-R503.
Bouck, D. and Bloom, K. (2005) The role of centromere-binding factor 3 (CBF3) in spindle stability, cytokinesis, and kinetochore attachment Biochem Cell Biology 83:696-702.
Bloom, K. (2004) Microtubule composition: Cryptography of dynamic polymers Proceedings of the National Academy of Sciences USA 101:6839-6840.
Pearson, C. G. and Bloom, K. (2004) Dynamic microtubules lead the way for spindle positioning Nature Reviews Cell and Molecular Biology 5:481-492.
Bloom, K. (2003) Microtubule Cytoskeleton: Navigating the Intracellular Landscape Current Biology, 13: R430–R432
Bloom, K., (2002)Yeast weighs in on the elusive spindle matrix: Proc. Natl. Acad. Sci. USA 99:4757-4759.
Segal, M., and Bloom, K. (2001) Control of Spindle Polarity and Orientation in Saccharomycescerevisiae. Trends in Cell Biology 11:160-166
Bloom, K. (2001) Nuclear Migration: Cortical anchors for cytoplasmicdynein. Current Biology 11:326-329
Bloom, K. (2000) It’s a kar9ochore to capture microtubules. Nature Cell Biology 2:96-98.
Bloom, K. and Beach, D.L. (1999). mRNA localization: Motile mRNA, asymmetric anchors. Current Opinion in Microbiology: Genetics and Development 2:604-609.
Bloom, K. (1993). The Centromere Frontier: Kinetochore components, microtubule-based motility and the Cen-value paradox.Cell 73: 621-624.
Schulman, I. and Bloom, K. (1991). Centromeres: An Integrated Protein/DNA Complex required for chromosome movement. Annual Review in Cell Biology Vol. 7:311-336.
Presentations:
Bloom, K. (2015) Physics of Chromosomes
Editorials:
Bloom, K. (2013) Intellectual Immigration Current Biology 23:R221-223.
Bloom, K. (2012) Nuclear Structure and Function Molecular Biology of the Cell 24:673
Mogilner, A., Edelstein-Keshet, L., and Bloom K. (2011) Guidelines for publishing papers containing theory and modeling Molecular Biology of the Cell 22:907-908